Coding

Part:BBa_K209406:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI C-D part, Beta Pix


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 420
    Illegal XbaI site found at 1842
    Illegal PstI site found at 575
    Illegal PstI site found at 841
    Illegal PstI site found at 1162
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 575
    Illegal PstI site found at 841
    Illegal PstI site found at 1162
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 724
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 420
    Illegal XbaI site found at 1842
    Illegal PstI site found at 575
    Illegal PstI site found at 841
    Illegal PstI site found at 1162
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 420
    Illegal XbaI site found at 1842
    Illegal PstI site found at 575
    Illegal PstI site found at 841
    Illegal PstI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 625
    Illegal BsaI.rc site found at 1927
    Illegal SapI.rc site found at 231


Design Notes

Cloned into pDONR221.

Source

References